To delete exons 2–10 of Trp53 in colonic stem cells, Trp53 flox/flox mice (Jackson, 008462) or Rosa26-LacZ mice (Jackson, 003474) were crossed with Lgr5-EGFP-IRES-creERT2 mice (Jackson, 008875; ref. 15). All mice were crossed onto a C57BL/6 background for at least 10 generations.
Long-term culture of adult progenitor cells in 3D is a recently emerging technology that inhabits the space between 2D cell lines and organ slice culture. Adaptations to defined media components in the wake of advances in ES and iPS cell culture has led to the identification of conditions that maintained intestinal cell progenitors in culture.
creERT2 mice were generated by homologous recombination in embryonic stem cells targeting an EGFP-IRES-creERT2 cassette to the ATG of created miR-34aflox/flox mice/Lgr5-GFP-IRES-CreERT2 mice. cells, we cultured mouse intestinal cells from Lgr5-EGFP-IRES-CreERT2 mice in 3D. Matrigel Mar 29, 2012 immunostaining in Lgr5-EGFP-IRES-CreERT2 and wild-type mice. We compared Lrig1 and Lgr5 expression, as Lgr5 is an established SC Apr 25, 2012 Mice lacking TLR4 in Lgr5-positive intestinal stem cells were gener- ated by first crossing the Lgr5-EGFP-IRES-creERT2 mice (12) with mT/mG. Jun 21, 2012 using the knock-in allele Lgr5EGFP-Ires-CreERT2, we Distribution of stem cells marked by Lgr5-GFP and Paneth cells, marked by lysozyme Lgr5陽性細胞でGFPとCreリコンビナーゼタンパク質を発現するLgr5-EGFP-Ires- CreERT2トランスジェニックマウスを、通称レインボーマウスと呼ばれる Sep 20, 2012 We visualized Lgr5-EGFP+ve cells in neonate Lgr5-.
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2010-01-08 · Lgr5-EGFP-ires-CreERT2 mice were injected with BrdU 2 hr prior to sacrifice in order to visualize actively cycling cells within the stomach. This revealed the presence of S-phase (i.e., cycling) cells within the Lgr5-EGFP +ve population at the gland base ( Figures 1 G and 1H; red arrows), although proliferation was generally less frequent than at the isthmus above the neck of the pyloric glands. 2018-01-09 · Lgr5-EGFP-Ires-CreERT2;R26R-Confetti double transgenic mice were treated with vehicle (n = 8) or G007-LK (n = 5) and tamoxifen according to the two administration schemes outlined in Fig. 1d, f. In control mice, lineage traced cells populated the entire length of the villi (Fig. 2 a) in the majority (69.9%) of the villi (Additional file 3 : Figure S2A). 2007-10-14 · Figure 4: EGFP expression in an Lgr5-EGFP-IRES-creERT2 knock-in mouse faithfully reproduces the Lgr5-lacZ expression pattern in the intestinal tract.
adult Lgr5-EGFP-ires-CreERT2/Rosa four-color mice. Confocal. analyses of the clonal output were performed at 14 days, 2 months, 6 months, and 12 months after the final tamoxifen.
May 8, 2014 GFP knockin allele: Yy1f/f;Vil-creERT2; Lgr5-EGFP-IRES-. creERT2 (43). Mice treated with tamoxifen and monitored for 4,.
2007-10-14 · Figure 4: EGFP expression in an Lgr5-EGFP-IRES-creERT2 knock-in mouse faithfully reproduces the Lgr5-lacZ expression pattern in the intestinal tract.
Following tamoxifen induction, it was possible to efficiently trace the progeny of Lgr5-expressing cells in gastrointestinal tissue via red fluorescent protein (RFP) expression.
By using the knock-in allele Lgr5 EGFP-Ires-CreERT2, we have shown that the cell surface receptor Lgr5 (leucine-rich repeat–containing heterotrimeric guanine nucleotide–binding protein–coupled receptor 5) marks normal tissue stem cells in stomach, small intestine, colon, and hair follicles . with Lgr5-eGFP-IRES-CreERT2 (Lgr5-Cre) mice. To this end, tamoxifen (Tam) was administered to 8-10-week-old mice. After three consecutive days of Tam treatment, double immunofluorescence staining showed that some Lgr5+ antral stem cells lacked E-cadherin expression in Lgr5-Cre;Cdh1fl/fl mice (Figure 2A). 南模生物. 利用CRISPR基因编辑技术，将EGFP-IRES-creERT2-WPRE-polyA插入到小鼠Lgr5基因起始密码子处。
Lgr5-eGFP-IRES-CreERT2 and Bmi1-CreER; Rosa26-YFP mice were treated with 12 Gy whole-body irradiation. By 2 d after irradiation, Lgr5-eGFP + ISCs and Olfm4 expression were completely lost from small intestine crypts ( Fig. 2 A , C , I – K , and L ), whereas there were no discernible quantitative effects on Bmi1-YFP + ISCs labeled with 1-d tamoxifen treatment ( Fig. 2 B and D ).
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Cre enzyme activity was induced by intraperitoneal injections of tamoxifen (2 mg/mouse). Murine small intestines To determine the cellular origin of CEA424-SV40-TAg tumours, we performed tracing experiments using Lgr5-EGFP-IRES-creERT2:CEA424-SV40-TAg:ROSA26-tdRFP mice. Following tamoxifen induction, it was possible to efficiently trace the progeny of Lgr5-expressing cells in gastrointestinal tissue via red fluorescent protein (RFP) expression. 2021-04-06 · in the case of intestinal organoids from Lgr5-EGFP-IRES-CreERT2 transgenic mice, in vitro expansion of Lgr5 expression is limited in a culture condition supplemented with essential proteins; NHE8 deficiency resulted in increased Lgr5 expression in the colon.
Transcriptional differences between ISCs and their daughter cells can be explored by use of the Lgr5‐EGFP‐ires‐CreERT2 knock‐in (Lgr5‐ki) mouse (Supplementary Figure S1A; Barker et al, 2007). In this mouse model, GFP expression is driven from the Lgr5 locus, leading to highest GFP levels in Lgr5 + cells (GFP high).
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Jan 15, 2021 Organoids from Lgr5-EGFP-ires-CreERT2-TdT mice were transplanted in C57Bl6 male mice exposed to PIR. Engraftment and repopulation of
Involvement Lgr5 RSCs in rectal epithelial regeneration was examined by lineage tracing assay. To this end, we first established a sorting strategy that would enable the isolation of pure populations of Lgr5 + E10.5-E12.5 hepatoblasts using the Lgr5-EGFP-IRES-creERT2 mouse line, where the eGFP reporter is knocked-in into the Lgr5 locus (Barker et al., 2007), combined with co-staining using an anti-Liv2 antibody, which specifically labels E9.5-E13.5 liver progenitors (Nierhoff et al Se hela listan på blog.csdn.net Lgr5-EGFP-IRES-creERT2基因敲入工具鼠 索取资料 欢迎您在此索取产品资料，联系订购产品，提交后您的需求信息将立即自动发送到供应商的E-mail邮箱，同时也将抄送一封到您的邮箱，感谢您的惠顾！ with WT Lgr5-EGFP-ires-creERT2 mice and by multiple generation selective breedings obtaining mice that were WT, CD44 /,or TLR4 / and heterozygous for the Lgr5-EGFP-ires-creERT2 re-porter. Mice were maintained on a 12-h:12-h light/dark schedule in a temperature-controlled specific pathogen-free facility and fed stan-dard laboratory mouse chow.